Molecular Biology 187 All prices are indicative and exclude VAT. Visit www.dulis.nl transport, storage and extraction of nucleic acids Qiacard FTA is a card impregnated with a patented chemistry: when a biological sample is deposited on the FTA, this chemistry lyses the cells and denatures the proteins, while the nucleic acids are extracted and protected in the paper fibres. The FTA card can be transported at room temperature to the laboratory or can be stored at room temperature for later analysis. hFor various samples: blood, cell cultures, oral cells, plant material, bacteria, plasma hStorage at room temperature or in the freezer for unstable samples (RNA) hAvailable with colour indicator, the deposit area changes colour to facilitate the location of the sample, recommended for colourless samples hNucleic acids ready for downstream application in less than 30 minutes hPrevents the growth of bacteria and fungi hProtects nucleic acids from environmental degradation hReduces the possibility of cross-contamination between samples hNucleic acid storage time: 17 years Apply the sample and let it dry Cutting a disc from the deposit area Place the disc in a PCR tube and wash 3 times with the purification reagent - discard the reagent after each wash Wash twice with TE-1 buffer (10 mM Tris, 0.1 mM EDTA, pH 8) and discard the used buffer after each wash Dry the disc in the PCR tube Add the PCR mix directly and amplify Qiacard: Qiacard FTA Kit for collect transport, storage and extraction of nucleic acids 036615 QIAcard FTA Elute Micro 40 4 25 N/A - 036820 QIAcard FTA Elute Micro 40 4 100 N/A - 610003 QIAcard FTA Elute Micro with indicator 40 1 25 N/A - 037069 QIAcard FTA Elute Micro with indicator 40 1 100 N/A - Qiacard FTA FTA purification reagents and accessories Cat. No. Description Units/ box €/ box WB120112 QIAcard FTA Wash Buffer, 25 ml 1 N/A - 036374 QIAcard FTA Wash Buffer, 500 ml 1 N/A - 036368 Resealable multi-barrier pouch for CloneSaver 17.8 x 18.7 cm 50 N/A - 610004 Transparent multi-barrier pouch 9.5 x 7.6 cm 100 N/A - 610005 Multi-barrier pouch 10.1 x 11.4 cm 100 N/A - 036398A Multi-barrier pouch 8 x 7 cm 100 N/A - 036376A Multi-barrier pouch 9 x 15 cm 100 N/A - 036399 Desiccant 1g moisture indicator 1000 N/A - WB100032 Swab with foam head for collection of oral samples, sterile 100 N/A - 036396A Swab with brush-like ejector head for taking oral samples, sterile 100 N/A - WB100073 Biopsy punch 1 mm 25 N/A - WB100074 Biopsy punch 1.2 mm 25 N/A - 037223 Biopsy punch 2 mm 25 N/A - 037227 Biopsy punch 3 mm 25 N/A - 037171 Biopsy punch 6 mm 25 N/A - WB100088 Cutting mat 6.3 x 7.6 cm 1 N/A - 036394 Cutting mat 15.2 x 20.3 cm 1 N/A - 036415 Kit including 4 x 1.2 mm biopsy punches and 2 cutting mats 1 N/A - 034988 Kit including 4 biopsy punches 2 mm and 2 cutting mats 1 N/A - 036826 Kit including 4 biopsy punches 3 mm and 2 cutting mats 1 N/A - 036899 Kit including 4 biopsy punches 6 mm and 2 cutting mats 1 N/A - Cat. No. Description Max. sample volume (µl) / circle Number of circles/ unit Units/ box €/ box 037067 QIAcard FTA Micro 125 1 25 N/A - 036370 QIAcard FTA Micro 125 1 100 N/A - WB120355 QIAcard FTA Mini 125 2 25 N/A - 036388 QIAcard FTA Mini 125 2 100 N/A - 036745 QIAcard FTA Classic 125 4 25 N/A - 036387 QIAcard FTA Classic 125 4 100 N/A - 036403 QIAcard FTA Gene 75 3 100 N/A - WB120311 QIAcard FTA Micro with indicator 125 1 25 N/A - 036372 QIAcard FTA Micro with indicator 125 1 100 N/A - 034987 QIAcard FTA Mini with indicator 125 2 25 N/A - 036375 QIAcard FTA Mini with indicator 125 2 100 N/A - 036749 QIAcard FTA Classic with indicator 125 4 25 N/A - 036360 QIAcard FTA Classic with indicator 125 4 100 N/A - 036367 QIAcard FTA CloneSaver 5 96 5 N/A - 036778 QIAcard FTA PlantSaver - - 100 N/A - Qiacard FTA with indicator Qiacard FTA CloneSaver hFor purification of plasmid DNA and bacterial DNA hWith colour indicator hStorage time of plasmid DNA: 4 years Qiacard FTA for plant samples FTA Elute Kit hFTA kit for collection, transport, storage and extraction of DNA at room temperature h12-40 µl per sample collected QIAcard FTA DMPK hBlood, plasma, urine or cerebrospinal fluid samples hFTA DMPK-A and FTA DMPK-B cards lyse cells and denature proteins. hThe FTA DMPK-C card is not impregnated with chemicals and does not denature proteins, suitable for protein-based biomolecules and antibody studies hOnly 10 - 20 µl of sample 037164 QIAcard FTA DMPK-A 10 - 20 3 100 N/A - 037195 QIAcard FTA DMPK-B 10 - 20 3 100 N/A - 037157 QIAcard FTA DMPK-C 10 - 20 3 100 N/A - 610006 Rack pour 20 QIAcards FTA DMPK - - 25 N/A - QIAcard Bloodstain hNon-indicator and non-FTA treated card, for collection, short term handling of samples hSamples of blood or other body fluids 036401 QIAcard Bloodstain 125 4 100 N/A -
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