Molecular Biology 200 Blotting, hybridization and revelation Detection and blocking For chemiluminescent signal detection of nucleic acid and protein blots. h Hyperfilm™ ECL hRapid detection of chemiluminescent signals from nucleic acid and protein blots hClear base with high contrast for easy reading of images hSensitive to blue and green light chemiluminescence systems hIdeal for use with ECL, ECL Plus, CDP-Star and other chemiluminescent systems h Hyperfilm™ MP hMulti-purpose film for fast detection of 14C, 35C, 33P, 32P, 125I hClear base with high contrast for easy reading of images hHigh resolution results hDedicated to direct and indirect autoradiography use Sheets size Cat. No. Cat. No. 18 x 24 cm 28-9068-36 50 N/A - 28-9068-43 50 N/A - 18 x 24 cm 28-9068-37 100 N/A - 28-9068-44 100 N/A - 8 x 10 inches 28-9068-38 50 N/A - 28-9068-45 50 N/A - 8 x 10 inches 28-9068-39 100 N/A - 28-9068-46 100 N/A - 5 x 7 inches 28-9068-35 50 N/A - 28-9068-42 50 N/A - €/ box €/ box Sheets/ box Sheets/ box Hyperfilm™ ECL Hyperfilm™ MP Amersham Hyperfilm™ ECL and MP Cat. No.. Reagents (volumes) Covered membrane area (cm2) € 051729 Luminol (25 ml) + peroxide solution (25 ml) 500 N/A - 051730 Luminol (50 ml) + peroxide solution (50 ml) 1000 N/A - 051731 Luminol (250 ml) + peroxide solution (250 ml) 5000 N/A - Immobilon Western LUMINOL chemiluminescence HRP substrates hPremixed chemiluminescence reagents for HRP detection in western blotting hNo risk of pipetting errors, no storage problems, ready to use hCover a wide range of sensitivities while being stable at room temperature or 4 °C Cat. No. Type Volume (mL) Detection range Signal duration € 053725 Luminata™ Classico Western 100 6 pg 1 hour N/A - 053726 Luminata™ Classico Western 500 6 pg 1 hour N/A - 053727 Luminata™ Crescendo Western 100 1 - 3 pg 3 hours N/A - 053728 Luminata™ Crescendo Western 500 1 - 3 pg 3 hours N/A - 053729 Luminata™ Forte Western 100 400 fg 3 hours N/A - 053730 Luminata™ Forte Western 500 400 fg 3 hours N/A - HRP Luminata™Substrates hHighly sensitive substrate hWide range of sensitivities, possibility to reduce the amount of antibody used hSignal duration over 2 hours hStable working solution for 10 days Signal duration › 2 hours Primary detection method X-ray or imaging system Typical antibody dilution Primary: 1: 1 000 - 1: 20000 Secondary: 1: 20 000 - 1: 200000 Stability ≥ 9 months from receipt (HRP: working solution: 10 days at 2 - 8 °C) Recommended initial exposure time 30 seconds (on film) Membrane compatibility PVDF, nitrocellulose or all commonly used buffers and blocking reagents commonly used Storage 2 to 8 °C
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