Molecular Biology

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Molecular Biology 184 Contents Locate the main types of applications for our products by following the pictograms: SAMPLE SPECIFIC APPLICATIONS PURIFICATION ELECTROPHORESIS INSTRUMENT MATERIALS Human Cloning Column Chemical Animal Genotyping Amplification Quantification NGS Magnetic purification Plant Micro-organism/virus Electrophoresis Instrument Material Decontamination of surfaces and instruments Collection, transport and storage of nucleic acid samples Nucleic acid electrophoresis Agarose Pre-cast gels Buffers Gel cutting accessories Blotting, hybridization and revelation Membranes and blotting papers for nucleic acids Membranes and blotting papers for proteins Revelation Accessories Reagents and chemicals for Molecular Biology and Biochemistry grades Alternatives to alkylphenol ethoxylate detergents 186 187 to 188 189 to 194 189 to 191 192 192 y 193 193 y 194 195 to 207 195 y 196 197 to 199 200 to 203 204 to 207 207 208

Molecular Biology 185 All prices are indicative and exclude VAT. Visit www.dulis.nl Véronique VAN DEN BOSSCHE is ready to help you with all your questions: quotation requests, offers & prices. Do not hesitate to contact her at +32 81 94 66 00 or by email veronique.vandenbossche@dulis.be WELCOME TO DULIS CUSTOMER SERVICE & PURCHASING Véronique VAN DEN BOSSCHE, Customer Care Coordinator. SERVICE, RESPONSIVENESS AND AVAILABILITY ARE PART OF OUR DAILY PRIORITIES. TO FOLLOW UP YOUR ORDERS, PRODUCTS AVAILABILITY, DEADLINES, please contact your advisor by email: orders@dulis.be ANY QUESTIONS? NEED INFORMATION? VISIT OUR WEBSITE www.dulis.be I www.dulis.nl OR CONTACT US VIA info@dulis.be

Molecular Biology 186 Decontamination before handling nucleic acids Enter Cat. No. at www.dulis.nl to compare DNA Exitus Plus to other reagents. WEB Cat. No. Description € 215445 250 ml Spray bottle N/A - 215446 500 ml Refill N/A - hHighly effective decontamination solution for the removal of DNA and RNA contamination from surfaces, instruments and laboratory equipment hIdeal for decontamination of PCR workplaces hCan also be used for decontamination of electrophoresis chambers, pipettes, reaction tubes, etc. h Advantages: ready to use, non-alkaline, non-carcinogenic Volume Spray botlle 049918 049920 - - € N/A - N/A - - - Flask - - 049922 049923 € HT - - N/A - N/A - 100 ml 500 ml 1 l 2.5 l DNA-ExitusPlus Volume Spray bottle 049927 049929 € N/A - N/A - 100 ml 500 ml DNA-ExitusPlus IF RNase-ExitusPlus Non-alkaline, non-corrosive, non-carcinogenic, highly active cleaning solution for RNase contamination. Stable for about 18 months and heat resistant. Biodegradable, non-toxic compounds. Mineral acids and alkaline substances that are not harmful to equipment even after long incubation times. A temperature of about 50 °C will increase the reaction rate. Store at room temperature. Volume Spray bottle 049912 - - € N/A - - - Flask - 049914 049915 € - N/A - N/A - 500 ml 1 l 2.5 l DNA-ExitusPlus For decontamination in 10 minutes at temperatures of 20 °C or above. For the decontamination of surfaces, equipment, plastics and glassware as well as pipettes. Simply apply the product to the surfaces to be decontaminated or dip the containers in the decontaminant. Instructions for use supplied with the product. Based on bio-degradable compounds. Incorporates a coloured indicator to identify the treated surface before wiping (IF version without indicator). DNA-ExitusPlus and DNA-ExitusPlus IF - RNase-ExitusPlus RNase Away spray bottle and flask DNA Away bottle Description Spray bottle 475 ml Bottle 250 ml Bottle 1 litre Cat. No 038184 038185 038186 038187 € N/A - N/A - N/A - N/A - Flask 4 litres Cat. No. Description 038188 250 ml bottle of DNA Away N/A - € Remove all traces of RNase and DNA! h RNase Away Ready to use, RNase Away solution avoids any risk of RNase contamination. In spray bottle or bottle. h DNA Away To remove all traces of DNA from PCR workstations, benches and equipment. Ready to use. RNase Away and DNA Away Decontaminant DNADecon

Molecular Biology 187 All prices are indicative and exclude VAT. Visit www.dulis.nl transport, storage and extraction of nucleic acids Qiacard FTA is a card impregnated with a patented chemistry: when a biological sample is deposited on the FTA, this chemistry lyses the cells and denatures the proteins, while the nucleic acids are extracted and protected in the paper fibres. The FTA card can be transported at room temperature to the laboratory or can be stored at room temperature for later analysis. hFor various samples: blood, cell cultures, oral cells, plant material, bacteria, plasma hStorage at room temperature or in the freezer for unstable samples (RNA) hAvailable with colour indicator, the deposit area changes colour to facilitate the location of the sample, recommended for colourless samples hNucleic acids ready for downstream application in less than 30 minutes hPrevents the growth of bacteria and fungi hProtects nucleic acids from environmental degradation hReduces the possibility of cross-contamination between samples hNucleic acid storage time: 17 years Apply the sample and let it dry Cutting a disc from the deposit area Place the disc in a PCR tube and wash 3 times with the purification reagent - discard the reagent after each wash Wash twice with TE-1 buffer (10 mM Tris, 0.1 mM EDTA, pH 8) and discard the used buffer after each wash Dry the disc in the PCR tube Add the PCR mix directly and amplify Qiacard: Qiacard FTA Kit for collect transport, storage and extraction of nucleic acids 036615 QIAcard FTA Elute Micro 40 4 25 N/A - 036820 QIAcard FTA Elute Micro 40 4 100 N/A - 610003 QIAcard FTA Elute Micro with indicator 40 1 25 N/A - 037069 QIAcard FTA Elute Micro with indicator 40 1 100 N/A - Qiacard FTA FTA purification reagents and accessories Cat. No. Description Units/ box €/ box WB120112 QIAcard FTA Wash Buffer, 25 ml 1 N/A - 036374 QIAcard FTA Wash Buffer, 500 ml 1 N/A - 036368 Resealable multi-barrier pouch for CloneSaver 17.8 x 18.7 cm 50 N/A - 610004 Transparent multi-barrier pouch 9.5 x 7.6 cm 100 N/A - 610005 Multi-barrier pouch 10.1 x 11.4 cm 100 N/A - 036398A Multi-barrier pouch 8 x 7 cm 100 N/A - 036376A Multi-barrier pouch 9 x 15 cm 100 N/A - 036399 Desiccant 1g moisture indicator 1000 N/A - WB100032 Swab with foam head for collection of oral samples, sterile 100 N/A - 036396A Swab with brush-like ejector head for taking oral samples, sterile 100 N/A - WB100073 Biopsy punch 1 mm 25 N/A - WB100074 Biopsy punch 1.2 mm 25 N/A - 037223 Biopsy punch 2 mm 25 N/A - 037227 Biopsy punch 3 mm 25 N/A - 037171 Biopsy punch 6 mm 25 N/A - WB100088 Cutting mat 6.3 x 7.6 cm 1 N/A - 036394 Cutting mat 15.2 x 20.3 cm 1 N/A - 036415 Kit including 4 x 1.2 mm biopsy punches and 2 cutting mats 1 N/A - 034988 Kit including 4 biopsy punches 2 mm and 2 cutting mats 1 N/A - 036826 Kit including 4 biopsy punches 3 mm and 2 cutting mats 1 N/A - 036899 Kit including 4 biopsy punches 6 mm and 2 cutting mats 1 N/A - Cat. No. Description Max. sample volume (µl) / circle Number of circles/ unit Units/ box €/ box 037067 QIAcard FTA Micro 125 1 25 N/A - 036370 QIAcard FTA Micro 125 1 100 N/A - WB120355 QIAcard FTA Mini 125 2 25 N/A - 036388 QIAcard FTA Mini 125 2 100 N/A - 036745 QIAcard FTA Classic 125 4 25 N/A - 036387 QIAcard FTA Classic 125 4 100 N/A - 036403 QIAcard FTA Gene 75 3 100 N/A - WB120311 QIAcard FTA Micro with indicator 125 1 25 N/A - 036372 QIAcard FTA Micro with indicator 125 1 100 N/A - 034987 QIAcard FTA Mini with indicator 125 2 25 N/A - 036375 QIAcard FTA Mini with indicator 125 2 100 N/A - 036749 QIAcard FTA Classic with indicator 125 4 25 N/A - 036360 QIAcard FTA Classic with indicator 125 4 100 N/A - 036367 QIAcard FTA CloneSaver 5 96 5 N/A - 036778 QIAcard FTA PlantSaver - - 100 N/A - Qiacard FTA with indicator Qiacard FTA CloneSaver hFor purification of plasmid DNA and bacterial DNA hWith colour indicator hStorage time of plasmid DNA: 4 years Qiacard FTA for plant samples FTA Elute Kit hFTA kit for collection, transport, storage and extraction of DNA at room temperature h12-40 µl per sample collected QIAcard FTA DMPK hBlood, plasma, urine or cerebrospinal fluid samples hFTA DMPK-A and FTA DMPK-B cards lyse cells and denature proteins. hThe FTA DMPK-C card is not impregnated with chemicals and does not denature proteins, suitable for protein-based biomolecules and antibody studies hOnly 10 - 20 µl of sample 037164 QIAcard FTA DMPK-A 10 - 20 3 100 N/A - 037195 QIAcard FTA DMPK-B 10 - 20 3 100 N/A - 037157 QIAcard FTA DMPK-C 10 - 20 3 100 N/A - 610006 Rack pour 20 QIAcards FTA DMPK - - 25 N/A - QIAcard Bloodstain hNon-indicator and non-FTA treated card, for collection, short term handling of samples hSamples of blood or other body fluids 036401 QIAcard Bloodstain 125 4 100 N/A -

Molecular Biology 188 transport, storage and extraction of nucleic acids For various samples: blood, plasma, oral cells, cell cultures, bacteria, urine, tissue lysate, liquid biopsy, purified DNA Available in two colours: • white for easily identifiable samples, such as blood • pink for colourless samples Card for collection, transport and storage of samples Cat. No. Description Colour Max. sample volume (µl) / circle Number of circles / unit Units/ box Features €/ box 175247 GenSaver 2.0 White 5 96 50 Preservation of nucleic acids at room temperature for up to 20 years, eliminates the risk of biological contamination of samples N/A - 175248 100 N/A - 175249 125 4 100 N/A - 175250 2 100 N/A - 175251 1 100 N/A - 175252 GenSaver 2.0 Pink 5 96 50 Preservation of nucleic acids at room temperature for up to 20 years, eliminates the risk of biological contamination of samples N/A - 175253 100 N/A - 175254 125 4 100 N/A - 175255 2 100 N/A - 175256 1 100 N/A - 175257 GenReleaz 2.0 White 125 4 100 Rapid collection, transport, storage and extraction of DNA at room temperature, storage of nucleic acids at room temperature for up to 20 years N/A - 175258 2 100 N/A - 175259 1 100 N/A - 175260 GenReleaz 2.0 Pink 125 4 100 Rapid collection, transport, storage and extraction of DNA at room temperature, storage of nucleic acids at room temperature for up to 20 years N/A - 175261 2 100 N/A - 175262 1 100 N/A - 175263 AutoCollect White 20 4 100 Sample storage for mass spectrometry analysis, for automated sample analysis, 2 years of nucleic acid storage N/A - 175264 AutoCollect Pink 10 4 100 N/A - 175265 AutoCollect with barcode White 20 4 100 N/A - 175266 Biological samples RUO White 70 5 100 In pure absorbent fibres, for storage and subsequent analysis of proteins or nucleic acids N/A - 175267 RUO biological samples with perforations 100 N/A - 175268 CE biological samples with perforations 100 N/A - 175269 RUO card without perforations White 70 5 100 In pure absorbent fibres, for storage and subsequent analysis of proteins or nucleic acids N/A - 175270 CE card without perforations 100 N/A - 175271 Card with no perforations 100 N/A - Cat. No. Description Units/ box €/ box 175272 Drying rack 10 N/A - Accessoire

Molecular Biology 189 All prices are indicative and exclude VAT. Visit www.dulis.nl agarose hUltra pure agarose with low background noise hEasy to dissolve hCan be used in low concentrations (0.75 - 2%) hIdeal for the separation of fragments from 250 bp to 25 kb hGel strength: < 1000 g/cm (1 %), > 2000 g/cm (1.5 %) hClarity: < 4 NTU Cat. No. Description € 523277 Agarose 100 g N/A - 523278 Agarose 500 g N/A - Cat. No. Description € 348376 Agarose 500 g N/A - DNase RNase hUsed for the separation of DNA and RNA fragments at concentrations ranging from 0.8 to 2% Molecular biology grade agarose powder Basic agarose Enter the Cat. No. on www.dulis.nl to find the Agarose selection guide WEB Cat. No. Type Applications Flask € 4905008 High.melting temp./ medium.resol.. Standard on DNA 25 g N/A - 4905009 High. melting temp./ medium. resol.. Standard on DNA 500 g N/A - Agarose

Molecular Biology 190 Agarose Features Packaging Type EEO Gel strength (g/cm2) Geling temperature Melting temperature Fragment size for analytical separation (bp) Applications 50 g 100 g 250 g 500 g 1000 g Cat. No. € Cat. No. € Cat. No. € Cat. No. € Cat. No. € D1 Low EEO 0.05 - 0.13 ≥ 1200 (1 %) 36± 1.5 °C (1.5 %) 88 ± 1.5 °C (1.5 %) ≥ 1000 High quality, low electroendosmosis agarose for nucleic acid electrophoresis - - 777001 N/A - 777002 N/A - 777003 N/A - 777004 N/A - D1 Medium EEO 0.16 - 0.19 ≥ 1000 (1 %) Medium electroendosmosis agarose for counter current electrophoresis, antibodies, serum and immunoelectrophoresis 778603 N/A - 777008 N/A - 777009 N/A - 777010 N/A - 777011 N/A - D1 High EEO 0.23 - 0.26 ≥ 750 (1 %) High electroendosmosis agarose for protein separation and countercurrent electrophoresis 778606 N/A - 777012 N/A - 777013 N/A - 777014 N/A - 777015 N/A - D1 LE GQT 0.05 - 0.13 ≥ 1200 (1%) Genetic engineering grade agarose tested for in-gel applications 778604 N/A - 777005 N/A - 777006 N/A - 777007 N/A - 778508 N/A - D2 ≤ 0.14 ≥ 900 (1 %) 42 ± 1.5 °C (1.5 %) 87 ± 1.5 °C (1.5 %) ≥ 1000 Agarose with high thermal stability and high melting temperature for derivatisation, crosslinking or coupling of enzymes, antigens or other substances to the gel structure 778607 N/A - 777016 N/A - 777017 N/A - 777018 N/A - 778509 N/A - D5 ≤ 0.12 ≥ 1800 (1 %) 36 ± 1.5 °C (1.5 %) 88 ± 1.5 °C (1.5 %) ≥ 1000 High gel strength agarose for blotting, pulsed field electrophoresis 778602 N/A - 777019 N/A - 777020 N/A - 777021 N/A - 778510 N/A - FP DNA ≤ 0.13 ≥ 1400 (1 %) 36 ± 1.5 °C (1.5 %) 88 ± 1.5 °C (1.5 %) ≥ 1000 Agarose for applications requiring the best batchto-batch reproducibility in terms of quality and performance, ideal for forensics and quality control 778601 N/A - 777022 N/A - 777023 N/A - 777024 N/A - 778515 N/A - E NC ≥ 1000 (1 %) 36 ± 1.5 °C (1.5 %) 88 ± 1.5 °C (1.5 %) 250-23 kb Standard agarose for routine electrophoresis 778565 N/A - 778566 N/A - 778519 N/A - 778567 N/A - 777531 N/A - LM ≤ 0.12 ≥ 500 (1.5 %) ≤ 24-28 °C (1.5 %) ≤ 65 °C (1.5 %) ≥ 1000 Agarose for high resolution electrophoresis, very low melting and gelling temperature, fragment up to 1000 bp 777025 N/A - 777026 N/A - 777027 N/A - 777028 N/A - 778511 N/A - LM GQT ≤ 0.12 ≥ 500 (1.5 %) ≤ 24-28 °C (1.5 %) ≤ 65 °C (1.5 %) ≥ 1000 Agarose qualified for in-gel applications (genetic quality), very low melting and gelling temperature, fragments up to 1000 bp 777029 N/A - 777030 N/A - 777031 N/A - 778600 N/A - 778516 N/A - LM SIEVE ≤ 0.10 ≥ 1000 (4 %) ≤ 35 °C (4 %) ≤ 65 °C (4 %) ≥ 1000 Agarose for high resolution electrophoresis, very low melting and gelling temperature, fragments up to 1000 bp, tested for in-gel applications 777032 N/A - 777033 N/A - 777034 N/A - 777035 N/A - 778517 N/A - NOVAGEL GQT ≤ 0.13 ≥ 800 (4 %) ≤ 35 °C (4 %) ≤ 65 °C (4 %) 50-1000 Agarose qualified for ingel applications (genetic engineering grade), very low melting and gelling temperature, fragments from 50 to 1000 bp 777036 N/A - 777037 N/A - 777038 N/A - 777039 N/A - 778518 N/A - MS4 ≤ 0.12 ≥ 500 (3 %) ≤ 31 °C (3 %) ≤ 76 °C (3 %) 150-500 Agarose for high resolution electrophoresis, for the separation of very small nucleic acid fragments (primers) and up to 500 bp 777046 N/A - 777047 N/A - 777048 N/A - 777049 N/A - 778514 N/A - MS6 ≤ 0.12 ≥ 800 (3 %) ≤ 35 °C (3 %) ≤ 75 °C (3 %) ≤ 1000 Agarose for high resolution electrophoresis, small DNA fragments and PCR products 777050 N/A - 777051 N/A - 777052 N/A - 777053 N/A - 778507 N/A - MS8 ≤ 0.12 ≥ 600 (1.5 %) ≤ 35 °C (3 %) ≤ 80 °C (3 %) ≤ 1000 Agarose for high resolution electrophoresis, fragments up to 1000 bp 777054 N/A - 777055 N/A - 777056 N/A - 777057 N/A - 778512 N/A - MS12 ≤ 0.12 ≥ 2000 (1.5 %) ≤ 40.5 °C (4 %) ≤ 93 °C (4 %) 50-1500 Agarose for high resolution electrophoresis, fragments up to 1500 bp 777058 N/A - 777059 N/A - 777060 N/A - 777061 N/A - 778513 N/A - Agarose selection guide

Molecular Biology 191 All prices are indicative and exclude VAT. Visit www.dulis.nl Agarose Agarose Cat. No. Description Units/ box €/ box 523275B Agarose tablet 100 N/A - hLow electroendosmosis (EEO) agarose for rapid DNA or RNA separations hSuitable for gels from 0.75% to 2% and fragment separations from 250 bp to 23 kb hInitial dissolution of the pellet for 2 min at room temperature hProvides time savings and reproducibility for the production of gels Agarose tablets hTablet sufficient for 50 ml of 1% agarose gel, with or without TAE/TBE buffer, pre-stained with Midori Green Advance hTablet to be dissolved in ultrapure water before microwave heating hAll-in-one solution, time saving and reproducibility in gel preparation hMidori Green Advance is a non-carcinogenic alternative to ethidium bromide for nucleic acid labelling hStorage at room temperature Cat. No. Description € 523393 75 agarose tablets, TBE and Midori Green Advance N/A - 523406 75 agarose tablets, TAE and Midori Green Advance N/A - 523407 100 agarose tablets and Midori Green Advance N/A - Agarose tablets with or without TAE/TBE buffer and Midori Green Advance hFor routine DNA and RNA electrophoresis, blotting compatible gels hTablet sufficient for 50 ml of 1% agarose gel in TAE or TBE buffer, prestained with Clear G hTablet to be dissolved in ultrapure water before microwave heating hAll-in-one solution, time saving and reproducibility in gel preparation hStorage at room temperature Clear G is a non-carcinogenic alternative to ethidium bromide for nucleic acid labelling (UV or blue light excitation). Cat. No. Description € 215413 Agarose tablets, TAE buffer and Clear G - box of 75 N/A - 215414 Agarose tablets, TBE buffer and Clear G - box of 75 N/A - Agarose tablets, TAE or TBE buffer and clear G hAgarose in flasks ready to use: mix the flask, heat in the microwave for 1 min. on maximum power, shake gently, microwave again for 30 sec. Repeat until the agarose is completely dissolved. hAgarose already contains a DNA fluorescence dye: Pronosafe (a non-carcinogenic alternative to ethidium bromide). Excitation wavelength 309, 419 or 514 nm and emission at 537 nm. Applications Typee Fragments Separation ≥ 1000 bp Fragments Separation ≤ 1000 bp Preparative electrophoresis High resolution electrophoresis Protein electrophoresis D1 • LM Sieve • • MS • • • Agarose in flasks

Molecular Biology 192 Pre-cast gels Pre-cast gel / Buffer hPrecast gel system 1% agarose with TAE, GelGreen-stained (non-mutagenic and non-cytotoxic BET alternative) hSeparation of fragments from 600 to 10 000 pb hGel thickness of 6 mm hCan be used with the RunOne tank hThe GelGreen allows for a revealing LED system as well as a simplified waste treatment procedure (disposal with biological waste) Gel size (cm) Landscape 11.7 x 5.6 cm Medium 11.7 x 8.9 cm Landscape 11.7 x 5.6 cm Portrait 6.5 x 9.5 cm Portrait 6.5 x 9.5 cm Well format 12 + 1 5 + 2 24 + 1 10 5 + 1 Well thickness 1 mm 1.5 mm 1 mm 1 mm 1.5 mm Volume per well 30 µl 3 x 75 µl / 2 x 112 µl 11 µl 15 µl 70 µl Multi-channel compatibility 12-channel format No 12-channel format No No Packaging 10 gels/box 5 gels/box 10 gels/box 10 gels/box 10 gels/box Cat. No. 039090 039091 039092 039093 039094 € N/A - N/A - N/A - N/A - N/A - EMBITEC pre-cast gels Nucleic acid electrophoresis buffer Solution electrophoresis buffers Washing pad Freeze-dried buffer Solution Preservation buffers Hybridization buffer Cat. No. Description € 348604 TAE 10X, BM grade, 1 l N/A - 348605 TAE 50X, BM grade, 1 l N/A - 091582 TBE 10X, BM grade, 500 ml N/A - 348613 TBE 10X, BM grade, 1 l N/A - Cat. No. Description € 702678 TBS 10X pH 7.4, BM grade, 6 x 1 l N/A - Cat. No. Description € 4905001 TBE 10X for 10 l preparation N/A - 4905005 SSC 10X for 10 l preparation N/A - Cat. No. Description € 348619 TE 1X pH 8.0, BM grade, 500 ml N/A - 091576B TE 1X pH 8.0, BM grade, 1 l N/A - 348618 TE 1X pH 8.0 low EDTA, BM grade, 500 ml N/A - 348617 TE 1X pH 8.0 low EDTA, BM grade, 1 l N/A - Cat. No. Description € 091568B SSC 20X, BM grade, 1 l N/A - Fast and easy to use Buffer for protein electrophoresis and blotting h1 bag makes 1 litre of 1X buffer hNo pH adjustment required hFast running buffer for high voltage electrophoresis (300 - 350 V); allows rapid separation of fragments of less than 5 kb (a conventional 30 min cycle can be completed in only 10 min) Powder bufferClearLine® for nucleic acid electrophoresis Cat. No. Description Composition pH Units/ box €/ box 257081 TAE buffer (TrisAcetate-EDTA) 40 mM Tris, 20 mM acetic acid, 1 mM EDTA 8.2 - 8.4 100 N/A - 257082 TBE Buffer (Trisborate-EDTA) 89 mM Tris, 89 mM boric acid, 2 mM EDTA 8.2 - 8.5 50 N/A - 257083 Fast running buffer - 8.1-8.5 100 N/A -

Molecular Biology 193 All prices are indicative and exclude VAT. Visit www.dulis.nl Concentrated TAE buffers for electrophoresis gels Buffer / Accessories TAE (Tris-Acetate-EDTA) buffer is used in agarose gel electrophoresis for applications requiring high resolution and low voltage, high molecular weight separation of double-stranded DNA. The solutions are aseptically microfiltered. Cat. No. 348605 TAE 50 x BM 1 l N/A - Description € Packaging Cat. No. Description Vol. (ml) € 523252 Tris acetate-EDTA 50X 500 N/A - Ready to use. Calibrated to provide clean strips. Cat.No. Description Vol. (ml) € 523253 Tris borate-EDTA 10X 500 N/A - Concentrated TBE buffer x 10 For molecular biology. Electrophoresis buffer. Cat. No. 523253 TBE 10 x BM 500 ml N/A - 348613 TBE 10 x BM 1 l N/A - Description € Packaging Concentrated TAE buffers Concentrated TBE buffers TBE Buffers Cat. No. 4905001 TBE 10 x One sachet for 10 litres concentration 1 x 1 x content: 89 mM Tris, 89 mM borate, 1 mM EDTA, pH 8.2 - 8.4 N/A - 4905005 SSC 10 x One sachet for 10 litres concentration 1 x 1 x content: 150 mM NaCl, 15 mM sodium citrate, pH 7.8 - 8.1 N/A - Final quantity Composition €/ box For maximum space saving. Dissolve in pure water to obtain the final concentration indicated. Buffer (power) Buffer powder sachet Cat. No. 091516 Boric acid BM 1 kg N/A - Description € Packaging Cat. No. Description Packaging € 348307 Ethylenediaminetetraacetic acid disodium salt 0.05 M 1000 ml N/A - Cat. No. 091572B TRIS BM 1 kg N/A - Description € Packaging Boric acid TRIS Ethylenediaminetetraacetic acid Buffers Accessories hExcision of clean, reproducible gel strips of 6 x 3 mm hSingle use to avoid contamination hDoes not scratch the surface of UV tables Cat. No. Description Number of reactions € 523337 Agarose gel strip cutter 50 N/A - Agarose gel strip cutter

Molecular Biology 194 Accessories Accessories hTo select and section DNA or RNA strips on agarose gels hIn single-use polystyrene hPrevents damage to transilluminator displays hL x W: 154 x 13 mm hPackaged in bags of 10 Cat. No. Description2 € 10 units 086667B Scalpel for agarose gels N/A - hNo need for a scalpel, one-handed operation hAutoclavable hPress the button to lower the gel and the tip hAvoid cross-contamination hCompatible with all 1000 µl pipettes (Eppendorf, Gilson) Cat. No. €/ bag Units/ bag 999068 4 x 1 250 N/A - 999070 6.5 x 1 250 N/A - Dimensions (mm) DNase RNase Suction tips for electrophoresis gel strips Scalpel for agarose gels

Molecular Biology 195 All prices are indicative and exclude VAT. Visit www.dulis.nl Blotting, hybridization and revelation Membranes and blotting papers for nucleic acids h Specifications Model Thickness Surface Absorption Applications 3MMCHR Thickness 0.34 mm Smooth Medium Blotting andchromatographypaper GB003 Thick 0.8 mm Smooth Medium Gel holder GB005 Extra thick 1.2 mm Smooth Strong Extra blotting semi dry General purpose paper with very high absorption. Blotting paper GB003 Cat.No. Opitran model €/ box Units/ box Dim. (mm) Type 034870 GB 003 70 x 100 Blotting paper, smooth, 0.8 mm 100 N/A - 034869 GB 003 110 x 140 Blotting paper, smooth, 0.8 mm 100 N/A - 034826 GB 003 300 x 600 Blotting paper, smooth, 0.8 mm 25 N/A - 034827 GB 003 580 x 600 Blotting paper, smooth, 0.8 mm 50 N/A - 034825 GB 003 100 x 100 Blotting paper, smooth, 0.8 mm 50 N/A - 034848 GB 003 150 x 150 Blotting paper, smooth, 0.8 mm 100 N/A - 034849 GB 003 150 x 200 Blotting paper, smooth, 0.8 mm 100 N/A - 034850 GB 003 160 x 180 Blotting paper, smooth, 0.8 mm 100 N/A - 034851 GB 003 200 x 200 Blotting paper, smooth, 0.8 mm 100 N/A - 034867B GB 003 200 x 250 Blotting paper, rough, 1.0 mm 100 N/A - 034855 GB 003 460 x 570 Blotting paper, smooth, 0.8 mm 100 N/A - hUsed to limit the risk of trapping air bubbles hRecommended for semi-dry protein blotting Blotting paper GB005 Cat.No. Opitran model €/ box Units/ box Dim. (mm) Type 034829 GB 005 200 x 200 Blotting paper, smooth, 1.5 mm 25 N/A - 034830 GB 005 580 x 580 Blotting paper, smooth, 1.5 mm 25 N/A - 034828 GB 005 150 x 150 Blotting paper, smooth, 1.5 mm 25 N/A - Excellent resistance in humid environments? optimised thickness and very good absorbency make Whatman 3MM Chr the paper of choice for all blotting applications. 3MM Chr: a 0.34 mm thick paper widely used in chromatography and electrophoresis. The absorption rate is 130 mm / 30 min. Cat. No. Dimensions Units/ box Rolls (mm x m) 036081 20 x 100 1 roll N/A - 036082 100 x 100 1 roll N/A - 036083 150 x 100 1 roll N/A - 036084 190 x 100 1 roll N/A - 036085 230 x 100 1 roll N/A - 036086 270 x 100 1 roll N/A - Sheets (mm x mm) 036562 150 x 200 100 N/A - 036344 200 x 200 100 N/A - 036088 315 x 355 100 N/A - 036347 460 x 570 100 N/A - 036090 580 x 680 100 N/A - €/ box 3MM CHR Paper Other papers Type Description Flow rate Format (cm) Cat.No. Units/ box €/ box 1 Chr Standard paper, high flow 130 mm 46 x 57 036340 100 N/A - (Ep: 0.18 mm) / 30 min 25 x 25 036341 100 N/A - 20 x 20 036342 100 N/A - 3 Chr Thick paper, ideal for 130 mm 46 x 57 036343 100 N/A - electrophoresis (Ep: 0.6 mm) / 30 min 17 Chr Very thick paper. Area 190 mm 46 x 57 036348 25 N/A - smooth (Ep: 0.2 mm) / 30 min 4 Chr High flow rate paper. 180 mm 46 x 57 036349 100 N/A - (Ep: 0.21 mm) / 30 min Whatman™blotting papers hModerately loaded nylon membranes hMembrane designed for Southern and Northern blots techniques, as well as colonies, and Dot/slots blots hFixing capacity (› 400 µg/cm2) hThis membrane is ideal for isotopic and non-isotopic detection techniques hUniform membrane (pore distribution and size) hTwo thresholds available: 0.2 µm or 0.45 µm for optimal retention of larger oligos and DNA fragments Cat.No. Nytran model €/ box Units/ box Dim. (mm) Type 034763 N 0.2 µm 200 x 3 m Rolls 1 N/A - 034764 N 0.2 µm 300 x 3 m Rolls 1 N/A - Cat.No. Nytran model €/ box Units/ box Dim. (mm) Type 034775 N 0.45 µm 200 x 3 m Rolls 1 N/A - 034776 N 0.45 µm 300 x 3 m Rolls 1 N/A - Nytran N nylon blotting membranes

Molecular Biology 196 Blotting, hybridization and revelation Membranes and blotting papers for nucleic acids hVery high positive charge, low background noise hHigher nylon density than with other membranes increases sample fixation capacity hExcellent symmetry: always remains flat hNytran Super Charge membrane binds nine times more molecules than a conventional nylon membrane hConsistent membrane morphology: good uniformity of nylon distribution and therefore of loads Cat.No. Nytran SuperCharge €/ box Units/ box Dim. (mm) Type 034779 0.45 µm 110 x 140 Sheets 10 N/A - 034798 0.45 µm 200 x 3 m Rolls 1 N/A - 034799 0.45 µm 300 x 3 m Rolls 1 N/A - Nytran Super Charge (SPC) Nylon Transfer Membrane h Hybond-N hResistant hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hFor radioactive applications hUV light fixing h Hybond-N+ hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hFor detection by radioactivity, chemiluminescence, chemifluorescence h Hybond-NX hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hDeveloped specifically for use with low volumes of hybridization buffer and high flow applications hGenerates little background noise h Hybond-XL hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hEffective fixation of small fragments hLoaded nylon for optimised signal-to-noise ratio when working with radioactively labelled samples Cat. No. Dimensions Pore size Membrane material € the roll Hybond-NX in neutral nylon, for more demanding applications Hybond-XL in nylon optimised for radio-labelled probes RPN303N 30 cm x 3 m 0.45 µm Nylon N/A - RPN203N 20 cm x 3 m 0.45 µm Nylon N/A - RPN303B 30 cm x 3 m 0.45 µm Positively charged nylon N/A - RPN203B 20 cm x 3 m 0.45 µm Positively charged nylon N/A - RPN303T 30 cm x 3 m 0.45 µm Nylon N/A - RPN203T 20 cm x 3 m 0.45 µm Nylon N/A - RPN303S 30 cm x 3 m 0.45 µm Positively charged nylon N/A - RPN203S 20 cm x 3 m 0.45 µm Positively charged nylon N/A - Hybond-N in neutral nylon Hybond-N + in positively charged nylon, versatile Other sizes available: please contact us. Amersham™ HYBOND blotting membranes

Molecular Biology 197 All prices are indicative and exclude VAT. Visit www.dulis.nl Blotting, hybridization and revelation Membrane and blotting paper for proteins Whatman and Amersham membranes merge into the Amersham optimised range. Amersham Protran nitrocellulose transfer membranes hThe Amersham Hybond P membrane is available in a 0.2 µm pore size h“Sandwich" membranes, ready to use hMembrane sizes have been revised to meet the needs of users Older range New range Use "Whatman Protran Amersham Hybond C" Amersham Protran Chemiluminescence "Whatman Optitran Amersham Hybond C extra" Protran Supported Hybridization and reprobing "Amersham Hybond ECL Amersham Hybond C super" Amersham Protran Premium Chemiluminescence and fluorescence Packaging Amersham Protran Protran Supported Amersham Protran Premium Pore size Size (mm) Units/ box Cat. No. €/ box Cat. No. €/ box Cat. No. €/ box 0.1 µm 100 x 100 10 10600077 N/A - 25 10600076 N/A - 102 x 133 10 10600126 N/A - 140 x 160 25 10600064 N/A - 200 x 200 10 10600046 N/A - 25 10600045 N/A - 150 x 4 m Roll 10600010 N/A - 200 x 4 m Roll 10600005 N/A - 300 x 4 m Roll 10600000 N/A - 0.2 µm 80 x 90 25 10600094 N/A - - - - - 100 x 100 10 10600085 N/A - 10600081 N/A - 25 10600084 N/A - 10600080 N/A - 102 x 133 10 10600125 N/A - 10600128 N/A - 140 x 160 25 10600063 N/A - 10600068 N/A - 10600066 N/A - 200 x 200 10 10600044 N/A - 10600054 N/A - 10600050 N/A - 25 10600043 N/A - 10600053 N/A - 10600049 N/A - 300 x 600 5 10600032 N/A - 10600037 N/A - 10600035 N/A - 150 x 4 m Roll 10600011 N/A - 10600019 N/A - 10600014 N/A - 200 x 4 m Roll 10600006 N/A - 10600017 N/A - 10600009 N/A - 300 x 4m Roll 10600001 N/A - 10600015 N/A - 10600004 N/A - 0.45 µm 80 x 90 25 10600093 N/A - 10600098 N/A - 10600096 N/A - 100 x 100 10 10600073 N/A - 10600083 N/A - 10600079 N/A - 25 10600072 N/A - 10600082 N/A - 10600078 N/A - 102 x 133 10 10600124 N/A - 10600127 N/A - 140 x 160 25 10600062 N/A - 10600067 N/A - 10600065 N/A - 200 x 200 10 10600042 N/A - - - 10600048 N/A - 25 10600041 N/A - 10600051 N/A - 10600047 N/A - 300 x 600 5 10600033 N/A - 10600036 N/A - 10600034 N/A - 150 x 4 m Roll 10600012 N/A - 10600020 N/A - 10600013 N/A - 200 x 4 m Roll 10600007 N/A - 10600018 N/A - 10600008 N/A - 300 x 4 m Roll 10600002 N/A - 10600016 N/A - 10600003 N/A - Sandwich 0.1 µm 80 x 90 10 10600116 N/A - 140 x 160 10 10600105 N/A - Sandwich 0.2 µm 80 x 90 10 10600115 N/A - 10600120 N/A - 10600118 N/A - 140 x 160 10 10600104 N/A - - - 10600107 N/A - Sandwich 0.45 µm 80 x 90 10 10600114 N/A - 10600119 N/A - 10600117 N/A - 140 x 160 10 10600103 N/A - 10600108 N/A - - -

Molecular Biology 198 Blotting, hybridization and revelation Membrane and blotting paper for proteins hReady-to-use solution for blotting applications hContains two sheets of 3MM CHR paper of appropriate size for each membrane sheet hThe membrane is to be placed on the anode side of the gel hThe 3MM CHR paper sheets cover each side of the entire hThe gel is ready for transfer Older range New range Use "Amersham Hybond P Whatman Westran CS" Amersham Hybond P Chemiluminescence, compatible with fast bleaching solvents Whatman Westran S Amersham Hybond SEQ Sequencing and solid phase analysis Amersham Hybond LFP Amersham Hybond LFP Fluorescence (low membrane autofluorescence) Packaging Amersham Hybond P Amersham Hybond SEQ Amersham Hybond LFP Pore size Size (mm) Units/ box Cat. No. €/ box Cat. No. €/ box Cat. No. €/ box 0.2 µm 80 x 90 25 10600101 N/A - - - 10600102 N/A - 100 x 100 10 10600089 N/A - - - - - 200 x 200 10 10600058 N/A - - - 10600060 N/A - 25 10600057 N/A - - - - - 260 x 600 5 10600039 N/A - 10600030 N/A - 10600040 N/A - 260 x 4 m Roll 10600021 N/A - - - 10600022 N/A - 0.45 µm 80 x 90 25 10600100 N/A - - - - - 100 x 100 10 10600087 N/A - - - - - 25 10600086 N/A - - - - - 140 x 160 25 10600069 N/A - - - - - 300 x 600 5 10600038 N/A - - - - - 150 x 4 m Roll 10600029 N/A - - - - - 300 x 4 m Roll 10600023 N/A - - - - - Sandwich 0.2 µm 80 x 90 10 10600122 N/A - - - 10600123 N/A - Sandwich 0.45 µm 80 x 90 10 10600121 N/A - - - - - 140 x 160 10 10600110 N/A - - - - - Blotting paper Gel (not included) Membrane Blotting paper Amersham Hybond PVDF transfer membranes h100% pure nitrocellulose membrane 0.1 µm for ‹ 7 kD molecules hHigh fixation, low background noise hHigh retention of small proteins: - the 0.2 µm membrane retains samples smaller than 20 KD - the 0.45 µm membrane is designed for molecules larger than 20 KD hExcellent protein stability on Protran (› 5 years) hNo pre-wetting with methanol: before transfer, a simple wetting with water is sufficient hExcellent stability of the membrane: allows many manipulations Cat.No. Protran NC model €/ box Units/ box Dim. (mm) Type 034714 BA 83 0.2 µm 82 Discs 50 N/A - 034735 BA 85 0.45 µm 25 Discs 100 N/A - 034738 BA 85 0.45 µm 25 Discs 1000 N/A - 034701 BA 85 0.45 µm 132 Discs 25 N/A - 034736 BA 85 0.45 µm 132 Discs 50 N/A - 034737 BA 85 0.45 µm 137 Discs 50 N/A - Protran NC nitrocellulose transfer membrane

Molecular Biology 199 All prices are indicative and exclude VAT. Visit www.dulis.nl Immobilon-FL (PVDF) transfer membranes Optimised for applications with fluorescence detection hSame fixation characteristics as Immobilon-P (PVDF, 0.45 µm) hVery low background noise in fluorescence detection in western blotting hCompatible with the most commonly used fluorescence reagents hCompatible with non-fluorescent detection reagents Immobilon Sandwiches (PVDF) Transfer Membranes hPre-cut hSupplied with buffer, separators and absorbent sheets hCompatible with pre-cast gels Immobilon-NC transfer membranes (cellulose esters) Immobilon-NC HAHY membranes made of cellulose esters with surfactants, which improves their wettability and facilitates their handling during the transfer process. Immobilon™-Ny+ transfer membranes For greater sensitivity during re-hybridisation. Immobilon-Ny+ membranes optimised for reliable and reproducible transfer, immobilisation, hybridisation and rehybridisation. ImmobilonTM Transfer membranes Immobilon-P (PVDF) transfer membranes Immobilon-E (PVDF) transfer membranes Made of polyvinylidene fluoride (PVDF)hydrophobic, optimised for binding proteins transferred from different types of gels. hIdeal substrate for immunodetection hUsing the rapid protocol developed by Merck Millipore, time savings of 2 hours: elimination of the blocking step in a western blot, reduction of the number and duration of washes, without compromising specificity or sensitivity Immobilon-PSQ (PVDF) transfer membranes Made of polyvinylidene fluoride (PVDF) for the transfer of low molecular weight proteins. Immobilon-PSQ membranes: for blotting of ‹ 20 kD proteins hHigh yields in protein sequencing hNo surfactant hDo not require a methanol membrane activation step hBetter protein retention and durability than nitrocellulose membranes hCan be moistened directly with transfer buffer hCompatible detection methods: chemiluminescence, chromogenic and radioactivity hCompatible with Coomassie Blue, Ponceau-S Red, Sypro Ruby and colloidal gold dyes hMembranes Cat. No. 044435 and 044436 are supplied with blotting paper sheets Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044430 IEVH00005 0.45 Roll 265 x 1875 1 N/A - 044431 IEVH07850 0.45 Sheets 70 x 84 50 N/A - 044432 IEVH08100 0.45 Sheets 80 x 100 10 N/A - 044433 IEVH09120 0.45 Sheets 90 x 120 10 N/A - 044434 IEVH10100 0.45 Sheets 100 x 100 10 N/A - 044435 IESN07852 0.45 Sheets 70 x 84 20 N/A - 044436 IESN08132 0.45 Sheets 85 x 135 20 N/A - 044437 IEVH07804 0.45 Sheets 70 x 84 4 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 051587 IPFL10100 0.45 Sheets 100 x 100 10 N/A - 051588 IPFL20200 0.45 Sheets 200 x 200 10 N/A - 051589 IPFL00010 0.45 Roll 265 x 3750 1 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 051590 IPSN07852 0.45 Sheets 70 x 84 20 N/A - 051591 IPSN08132 0.45 Sheets 85 x 135 20 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044067 INYC00010 0.45 Roll 300 x 3300 1 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044055 HATC00010 0.45 Roll 330 x 3000 1 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044088 ISEQ00010 0.2 Roll 265 x 3750 1 N/A - 044089 ISEQ15150 0.2 Sheets 150 x 150 10 N/A - 044090 ISEQ101000 0.2 Sheets 100 x 100 10 N/A - 044091 ISEQ20200 0.2 Sheets 200 x 200 10 N/A - 044092 ISEQ26260 0.2 Sheets 260 x 260 10 N/A - Blotting, hybridization and revelation Membrane and blotting paper for proteins Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044081 IPVH07850 0.45 Sheets 70 x 84 50 N/A - 044082 IPVH304F0 0.45 Sheets 260 x 260 10 N/A - 044083 IPVH09120 0.45 Sheets 90 x 120 10 N/A - 044084 IPVH10100 0.45 Sheets 100 x 100 10 N/A - 044085 IPVH20200 0.45 Sheets 200 x 200 10 N/A - 044086 IPVH15150 0.45 Sheets 150 x 150 10 N/A - 044087 IPVH00010 0.45 Roll 265 x 3750 1 N/A -

Molecular Biology 200 Blotting, hybridization and revelation Detection and blocking For chemiluminescent signal detection of nucleic acid and protein blots. h Hyperfilm™ ECL hRapid detection of chemiluminescent signals from nucleic acid and protein blots hClear base with high contrast for easy reading of images hSensitive to blue and green light chemiluminescence systems hIdeal for use with ECL, ECL Plus, CDP-Star and other chemiluminescent systems h Hyperfilm™ MP hMulti-purpose film for fast detection of 14C, 35C, 33P, 32P, 125I hClear base with high contrast for easy reading of images hHigh resolution results hDedicated to direct and indirect autoradiography use Sheets size Cat. No. Cat. No. 18 x 24 cm 28-9068-36 50 N/A - 28-9068-43 50 N/A - 18 x 24 cm 28-9068-37 100 N/A - 28-9068-44 100 N/A - 8 x 10 inches 28-9068-38 50 N/A - 28-9068-45 50 N/A - 8 x 10 inches 28-9068-39 100 N/A - 28-9068-46 100 N/A - 5 x 7 inches 28-9068-35 50 N/A - 28-9068-42 50 N/A - €/ box €/ box Sheets/ box Sheets/ box Hyperfilm™ ECL Hyperfilm™ MP Amersham Hyperfilm™ ECL and MP Cat. No.. Reagents (volumes) Covered membrane area (cm2) € 051729 Luminol (25 ml) + peroxide solution (25 ml) 500 N/A - 051730 Luminol (50 ml) + peroxide solution (50 ml) 1000 N/A - 051731 Luminol (250 ml) + peroxide solution (250 ml) 5000 N/A - Immobilon Western LUMINOL chemiluminescence HRP substrates hPremixed chemiluminescence reagents for HRP detection in western blotting hNo risk of pipetting errors, no storage problems, ready to use hCover a wide range of sensitivities while being stable at room temperature or 4 °C Cat. No. Type Volume (mL) Detection range Signal duration € 053725 Luminata™ Classico Western 100 6 pg 1 hour N/A - 053726 Luminata™ Classico Western 500 6 pg 1 hour N/A - 053727 Luminata™ Crescendo Western 100 1 - 3 pg 3 hours N/A - 053728 Luminata™ Crescendo Western 500 1 - 3 pg 3 hours N/A - 053729 Luminata™ Forte Western 100 400 fg 3 hours N/A - 053730 Luminata™ Forte Western 500 400 fg 3 hours N/A - HRP Luminata™Substrates hHighly sensitive substrate hWide range of sensitivities, possibility to reduce the amount of antibody used hSignal duration over 2 hours hStable working solution for 10 days Signal duration › 2 hours Primary detection method X-ray or imaging system Typical antibody dilution Primary: 1: 1 000 - 1: 20000 Secondary: 1: 20 000 - 1: 200000 Stability ≥ 9 months from receipt (HRP: working solution: 10 days at 2 - 8 °C) Recommended initial exposure time 30 seconds (on film) Membrane compatibility PVDF, nitrocellulose or all commonly used buffers and blocking reagents commonly used Storage 2 to 8 °C

Molecular Biology 201 All prices are indicative and exclude VAT. Visit www.dulis.nl Blotting, hybridization and revelation Detection and blocking Cat. No. Description € 053777 Luminata Forte Elisa HRP Substrate 100 ml N/A - 053778 Luminata Crescendo Elisa HRP Substrate 100 ml N/A - 053779 Luminata Crescendo Elisa HRP Substrate 200 ml N/A - Luminata Elisa HRP Amersham ECL Select™ Amersham ECL Start™ Amersham ECL™ Amersham ECL Prime™ Contains: hECL Plex Goat - mouse IgG-Cy3, 150 µg hECL Plex Goat - rabbit IgG-Cy5, 150 µg hHybond ECL 10 x 10 cm (cdt 10) or Hybond-LFP 20 x 20 cm (cdt 3) hMarqueurs ECL Plex Rainbow 120 µl Amersham ECL Plex™: for quantitative detection Use of 2 fluorescent markers allowing the simultaneous detection of 2 proteins on western blots. Amersham ECL™ Amersham ECL Start™ Amersham ECL Prime™ Amersham ECL Select™ Amersham ECL Plex™ Kit features Routine Western blotting Confirmatory Western blotting for high to moderate sensitivity proteins Quantitative and confirmatory analysis High to low sensitivity proteins Quantitative and confirmatory analysis Medium to very low protein sensitivity Quantitative analysis Sensitivity (model system) ~10 pg N/C ~1 pg ~5 ng ~1.2 pg Primary antibody (model system) 1:100 - 1:5000 1:500 - 1:3000 1:1000 - 1:50000 1:5000 - 1:30000 1:100 - 1:5000 Secondary antibody (model system) 1:1000 - 1:15000 1:5000 - 1:50000 1:50000 - 1:250000 1:100000 - 1:300000 1:1250 - 1:4000 Emission duration 1 to 2 hrs 3 hrs 3 hrs 2 hrs › 3 months Reprobing with 2 antibodies Yes Yes Yes Yes Not required Recommended membrane Amersham Hybond P Amersham Protran Premium Amersham Hybond P Amersham Protran Amersham Hybond P Amersham Protran Premium Amersham Hybond Amersham Protran Premium Amersham Hybond LFP Amersham Protran Premium Recommended detection method Hyperfilm ECL and imageQuant imagers Amersham Hyperfilm and CCD camera detection range Hyperfilm ECL and imagers imageQuant Hyperfilm ECL and imagers imageQuant Fluorescence scanner and ImageQuant imagers hUses CyDye™ technology hMultiplex analysis of proteins Cat. No. Description € RPN3243 ECL Start for western blot 200 ml N/A - RPN3244 ECL Start for western blot 400 ml x 2 N/A - Cat. No. Description € RPN2108 ECL western blotting system for 1000 cm2 of membrane: anti-mouse HRP conjugate 100 µl, anti-Rabbit conjugate HRP 100 µl, detection reagents 1 and 2, blocking agent4 N/A - RPN2109 ECLWestern Blotting Detection Reagents for 1000 cm2 of membrane4 N/A - RPN2209 ECLWestern Blotting Detection Reagents for 2000 cm2 of membrane5 N/A - RPN2106 ECLWestern Blotting Detection Reagents for 4000 cm2 of membrane6 N/A - 4 contains 2 flasks of 62.5 ml 5 contains 2 flasks of 125 ml 6 contains 2 flasks of 250 ml Cat. No. Description € RPN2232 ECL PrimeWestern Blotting Reagent for 1000 cm2 of membrane1 N/A - RPN2236 ECL PrimeWestern Blotting Reagent for 3000 cm2 of membrane2 N/A - 1 contains 2 flasks of 50 ml 2 contains 2 flasks of 150 ml Cat. No. Description € RPN999 ECL Plex: CY3,CY5, RainbowMarker and HYBOND-LFP 20 x 20 cm N/A - Cat. No. Description € RPN2235 ECL Select Western blotting Reagent for 1000 cm2 of membrane3 N/A - 3 contains 1 x 50ml luminol flask and 1 x 50 ml peroxyde solution flask Protein labelling and detection Amersham ECL™, ECL Start™, ECL Advance™, ECL Select™, ECL Prime, hHRP reagent for chemoluminescent detection in ELISA format hRequires a suitable plate reader hHigh sensitivity to detect up to 0.1 pg of protein hWide dynamic range allowing a large number of detection thresholds hFlexible use, with two types of substrates, Luminata crescendo and Forte

Molecular Biology 202 Blotting, hybridization and revelation Detection and blocking Cat. No. Description PA45009 ECL Plex GaM IgG, CY5, 150 µg N/A - PA45011 ECL Plex GaR IgG, CY5, 150 µg N/A - PA43009 ECL Plex GaM IgG, CY3, 150 µg N/A - 28-9011-06 ECL Plex GaR IgG Cy3 150 µg N/A - € hApplications: Western blot hRevelation: ECL hStorage: 2 - 8 °C Cat.No. Description Volume (ml) € NA9340-1ML HRP conjugated Anti-rabbit IgG F(ab') 2 1 N/A - HRP conjugated Anti-rabbit IgG, F(ab’)2 Antibodies ECL Plex GaM IgG Consult us for secondary HRP-conjugated antibodies TIP hHigh quality with blue background hFor use with calcium tungstate or blue emitting screens hCompatible with manual and automatic development methods hThe UHC version offers higher contrast and sensitivity with low noise Cat.No. Version Dimensions L x W (mm) Units/ box €/ box 062632 Blu-Blot HS 127 x 178 100 N/A - 062633 Blu-Blot HS 203 x 254 100 N/A - 062666 Blu-Lite UHC 127 x 178 100 N/A - 062667 Blu-Lite UHC 203 x 254 100 N/A - Blu-Blot HS and Blu-Lite UHC autoradiography films hBlue background hSingle emulsion hWith intersheets hExceptional clarity and low background noise hFor use with 14C, 35S, 32P et 125I hBlotting hSequencing hChemiluminescence Autoradiography hShift gel analysis Cat. No. 789173 12.7 x 17.78 N/A - 789174 20.32 x 25.4 N/A - 789175 25.4 x 30.48 N/A - 789176 35.56 x 43.18 N/A - W x L (cm)2 € 100 units “Blue devil" autoradiography films For fast and highly sensitive results in the detection o 32P and 125I in Southern, Northern and Western blots. To be used with the pre-flash lamp. Cat. No. Dimensions Units/ box €/ box RPN1662 18 x 24 cm 2 N/A - Amersham Hyperscreen Intensification Screens Technique based on direct labelling of DNA or RNA probes with a thermostable alkaline phosphatase. This labelled probe is then hybridised to the target. hDetection possible by CDP-Star or ECF h2 to 3 hours reduction compared to conventional indirect methods Cat. No. Description Membrane Area RPN3680 Alkphos direct labeling module 2500 cm2 N/A - RPN3682 CDP-star detection reagent 2500 cm2 N/A - RPN3685 ECF dectection module 2500 cm2 N/A - RPN3688 Alkphos direct hybridisation buffer 5000 cm2 N/A - € Cytiva Amersham AlkPhos Direct™ direct marking and detection systems

Molecular Biology 203 All prices are indicative and exclude VAT. Visit www.dulis.nl ECL Prime blocking reagent Blotting, hybridization and revelation Detection and blocking Nucleic acid blotting The use of a synthetic blocking buffer reduces cross-reactions and background, especially for low abundance proteins. hReady to use Bløk blocking pads, protein free hStable at room temperature hAvailable in optimised versions for studying protein phosphorylation or working with fluorescent detection hCompatible with staining after immunodetection Cat. No. Type € per 500 ml 053731 CH reagent without background noise for detection N/A - by chemiluminescence 053732 FL reagent without background noise for detection N/A - by fluorescence 053733 Background-free PO reagent for the detection of N/A - phosphoproteins by chemiluminescence or fluorescence Cat. No. Description € 051965 Reblot plus Mild Solution 50 ml N/A - 051979 Reblot plus Strong Solution 50 ml N/A - These kits contain all the reagents for easy removal of antibodies after chemiluminescence antibody detection. hSimple and quick (10 to 20 minutes) hUse at room temperature hNo beta-mercaptoethanol h2 different solutions depending on the strength of the interaction between antibody and antigen Reblot Solutions BLOCK blocking reagents for chemiluminescence or fluorescence detection Cat. No. Description € RPN2125 ECL BLOCKING AGENT - 40 g N/A - hBlocking agent compatible with ECL reagents hFor use on PVDF or nitrocellulose membranes hBlocking agent compatible with ECL Prime and ECL Plex detection reagents Cat. No. Description € RPN418 ECL Prime blocking reagent 40 g N/A - ECL blocking agent hKit for rapid transfer with high resolution of DNA and RNA hFast: top-down transfer in one hour with alkaline buffers and three hours with neutral buffers (SSC) hEconomical: The buffer holder requires less buffer and blotting paper hEasy to use, no vacuum or power source required hSystem using gravity in addition to capillary action hCompact: small and stackable unit Each TurboBlotter kit includes: • 1 transfer system; • membranes and papers needed for 5 transfers. Each TurboBlotter refill includes the membranes and papers needed for 5 transfers. Cat. No. Description € Dim. (mm) Transfer syst. size 034800 Nytran SuperCharge TurboBlotter Kit 100 x 150 12 x 16 cm N/A - 034801 Nytran SuperCharge TurboBlotter Refill 100 x 150 12 x 16 cm N/A - 034802 Nytran SuperCharge TurboBlotter Kit 110 x 140 12 x 16 cm N/A - 034803 Nytran SuperCharge TurboBlotter Refill 110 x 140 12 x 16 cm N/A - 034807 Nytran SuperCharge TurboBlotter Refill 150 x 150 21 x 26 cm N/A - 034808 Nytran SuperCharge TurboBlotter Kit 150 x 200 21 x 26 cm N/A - 034809 Nytran SuperCharge TurboBlotter Refill 150 x 200 21 x 26 cm N/A - 034812 Nytran SuperCharge TurboBlotter Kit 200 x 250 21 x 26 cm N/A - 034813 Nytran SuperCharge TurboBlotter Refill 200 x 250 21 x 26 cm N/A - 034814 Nytran SuperCharge TurboBlotter Kit 70 x 100 12 x 16 cm N/A - Rapid transfer system for Southern and Northern blot: TurboBlotter

Molecular Biology 204 Blotting, hybridization and revelation Accessories hFor maximum contact between the sample and the film hCan be used down to -70 °C hSmooth, non-porous internal mat ensures minimal contamination hThe 1.5 cm thick model is used with thicker samples Cat. No. Dimensions € Model Colour RPN11649 Standard Neutral 8 x 10 inches N/A - RPN11650 Standard Neutral 10 x 12 inches N/A - Amersham autoradiography cassettes Regilait milk powder is ideal for saturating transfer membranes during Slot-Blot or Western Blot. In 750 g or 300 g tins with spout. Regilait skimmed milk Cat. No. Packaging € 711160 750 g N/A - 731142 300 g N/A - hXylene-based, permanent writing hFor nitrocellulose and nylon membranes hIdeal marker for orientation of membranes in different types of transfers Cat. No. 034982 Membrane marker pen 10 N/A - Description €/ box Units/ box Membrane marker pen hTo annotate the transfer membranes on PVDF or nitrocellulose hReacts with HRP substrates hFast and sensitive hCompatible with X-ray film and CCD imagers Cat. No. Description € 701100 Pre-stained chemiluminescent marker for Western blot N/A - Pre-stained chemiluminescent marker for Western blot hSpecial laboratory hInterchangeable fine tip for autoradiography hContains enough phosphor to be visible at different wavelengths Cat. No. 053397 Fluorescent marker with 4 tips N/A - Description2 € Fluorescent marker Cat. No. 029181 10 x 10 cm 125 x 125 x 50 N/A - For gels € L x W x H (mm) Gel staining boxes with drain valve Transparent boxes designed for staining and destaining of electrophoresis gels. hWaterproof cover hDrain valve allowing the transfer of liquid without movement hCarrying handles hStackable hTransparent PCTG boxes hFor staining, destaining, gel transport, membrane blotting

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