12 Molecular Biology MOLECULAR BIOLOGY Fast and easy to use h1 bag makes 1 litre of 1X buffer hNo pH adjustment required hFast running buffer for high voltage electrophoresis (300 - 350 V); allows rapid separation of fragments of less than 5 kb (a conventional 30 min cycle can be completed in only 10 min) Powder bufferClearLine® for nucleic acid electrophoresis Cat. No. Description Composition pH Units/ box € excl. VAT/box 257081 TAE buffer (Tris-AcetateEDTA) 40 mM Tris, 20 mM acetic acid, 1 mM EDTA 8.2 - 8.4 100 NC - 257082 TBE Buffer (Tris-borateEDTA) 89 mM Tris, 89 mM boric acid, 2 mM EDTA 8.2 - 8.5 50 NC - 257083 Fast running buffer - 8.1-8.5 100 NC - Fast and easy to use ClearLine® Powder buffers for protein electrophoresis h1 bag makes 1 litre of 1X buffer hNo pH adjustment required hMES-SDS buffer: ideal for low molecular weight proteins hMOPS-SDS buffer: ideal for high molecular weight proteins Cat. No. Description Composition pH Units/ box € excl. VAT/ box 257084 TGS Buffer (Tris-Glycine-SDS) 25 mM Tris, 192 mM Glycine, 0,1 % SDS 8.1 - 8.5 50 NC - 257085 MES-SDS Buffer 50 mM Tris, 50 mM MES, 0,1% SDS, 1 mM EDTA 6.8 - 7.2 50 NC - 257086 MOPS-SDS Buffer 50 mM Tris, 50 mM MOPS, 0,1% SDS, 1 mM EDTA 7.5-7.9 50 NC - 257087 PBS Buffer 137 mM NaCl, 2,7 mM KCl, 10 mM phosphate 7.2 - 7.6 100 NC - 257088 PBS-Tween Buffer 137 mM NaCl, 2,7 mM KCl, 10 mM phosphate, 0,05 % Tween-20 7.2 - 7.6 100 NC - 257089 TBS Buffer 137 mM NaCl, 2,7 mM KCl, 50 mM Tris 7.25 - 7.55 100 NC - 257090 TBS-Tween Buffer 137 mM NaCl, 2,7 mM KCl, 50 mM Tris, 0,1 % Tween-20 7.25 - 7.55 100 NC - hTo annotate the transfer membranes on PVDF or nitrocellulose hReacts with HRP substrates hFast and sensitive hCompatible with X-ray film and CCD imagers Cat. No. Description € excl. VAT 701100 Pre-stained chemiluminescent marker for Western blot NC - Pre-stained chemiluminescent marker for Western blot
RkJQdWJsaXNoZXIy MTUwNzIy