Molecular Biology - Nucleic Acids Extraction of nucleic acids by chemical method Extraction of DNA and RNA

Extraction of DNA and RNA

DNA extraction kit - ExtraCLEAR

• DNA extraction from various samples (bacteria, tissue, plants, organs, bird feathers, saliva)
• Protocole simple et rapide (8 minutes)
• Once the reagent has been mixed with the sample, it is simply incubated at 3 different temperatures (65°C 6 min, 98°C 2 min, 4°C)
• Extraction effectuée dans un themocycleur ou un bloc chauffant
• Non-toxic reagent
• Extracted DNA ready for PCR amplification

Arcis DNA Preparation KIT

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• DNA preparation in 3 min
• From blood, saliva, bacterium and cells samples or plant/animal tissues
• For later use in PCR, sequencing and cloning
• No purification required
• No incubation or centrifugation stage required
• Stable prepared DNA for 30 days stability at room temperature
• Also appropiate for RNA, by adding DNasea treatment stage

BR Plasmid Kit

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• Plasmid DNA insulation from Gram-negative and Gram-positive bacteria
• Low or high copy
• Fast and simple procedure
• Extraction/Purification: approx. 15 - 20 min.
• Pure plasmid DNA suitable for enzymatic reactions and sequencing

Cytiva illustra™ nucleic acid purification

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• illustra RNAspin 96 RNA insulation kit
• For a fast and efficient insulation of total RNA in 96-well format
• 96 samples can be parallel-processed in under 70 min
• DNase and prefilter plate included in the kit
• Up to 100 µg RNA yield, from 30 mg tissue
• Processing via either centrifugation or vacuum-based protocols
• 1 x 10⁷cells sample for cell culture centrifuge
• 2 x 10⁶cells sample for cell culture vacuum
• A 260 /A 280 between 1.8 and 2.2