Standard Routine DNA polymerases - Laboratoriumuitrusting

Cytiva PCR components

Taq DNA Polymerase (cloned): 1 unit incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 min at 70ºC, in a total volume of 50 µl.

Cytiva illustra™ PCR kits: Ready-To-Go™ technique

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PuReTaq Ready-To-Go PCR Beads are pre-mixed, predispensed, single-dose reactions optimised for performing standard PCR amplifications.

Routine enzyme

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• Fragment amplification up to 4 kb fidelity comparable to Taq, suitable for TA-cloning

Routine enzyme

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• Fragment amplification up to 4 kb fidelity comparable to Taq, suitable for TA-cloning

Thermoprime

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• Amplification of fragments from 12 kb
• Allows to make 4000 reactions of 25 µl each

Thermo-Start DNA Taq Polymerase

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• Requires an activation step at 95 °C for 15 minutes
• Inactive at room temperature
• Amplifying fragments up to 5 kb
• Contains
• Thermo start (5U/µl) 10 x 50 µl
• Buffer (10X) 10 x x1.5 ml
• MgCl₂ (25 mm) 10 x 1.5 ml

Thermoprime ReddyMix™ MasterMix

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Developed to minimize preparation time for the PCR reaction.
Combines the properties of ReddyMix to the effectiveness of the Thermoprime.
As MasterMix, available in multiple versions.

Taq DNA Polymerase Kit (Thermus aquaticus) 5 U/µl with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 5 U/µl
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl₂ (A), with MgCl₂ (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

Color Taq DNA Polymerase Kit (1 U/µl) with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 1 U/µl
• Contains 2 inert dyes (red and yellow) to facilitate PCR preparation and allow direct deposition on electrophoresis gel and migration monitoring
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 2 different 10X reaction buffers: without MgCl₂ (A) and with MgCl₂ (B)
• Kit supplied with dNTPs (separate tube)

Master mix SG qPCR (2X) and Master mix SG qPCR (2X) with ROX solution

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• SYBR Green I dye qPCR mix for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer, dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix Blank qPCR (2X) and Master mix Blank qPCR (2X) with ROX solution

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• Dye qPCR mix for use with most real-time thermal cyclers
• Contains no dye to allow choice of dye and optimisation of its concentration
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer and dNTPs (dTTP partially replaced by dUTP)
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix Probe qPCR (2X) and Master mix Probe qPCR (2X) with ROX solution

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• QPCR mix with hydrolysis probes for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase and optimised buffer and dNTPs (dTTP partially replaced by dUTP)
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Hi-fi Pop DNA polymerase

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• For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis
• Fidélité 60 fois supérieure à celle d'une Taq polymérase classique
• Amplification of long, GC-rich DNA sequences
• Les matrices complexes (très riches en GC, structures secondaires complexes, séquences très longues...), peuvent nécéssiter l'ajout d'enhancer bétaïne 5M (en option)
• High elongation rate: 10 sec/kb
• 3' -' 5' exonuclease activity