Taq polymérases, kits et master mixes

Disco taq DNA polymerase

• High throughput and reliable amplification for routine applications, screening or high-throughput screening
• Amplification jusqu'à 5 kb, compatible avec le TA-cloning et l'incorporation de dUTP
• 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity

Hot Disco Taq DNA polymerase

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• Inactivated at room temperature, activation at 95°C for 15 min
• Incorporation possible de dUTP
• High specificity and sensitivity for high performance
• Pour détection de cibles même en faible quantité, le screening, le mutliplexage, la PCR directe sur colonies, et les applications de qPCR

Samples available, please contact us!

Taq DNA Polymerase Kit (Thermus aquaticus) 5 U/µl with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 5 U/µl
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl₂ (A), with MgCl₂ (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

Color Taq DNA Polymerase Kit (1 U/µl) with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 1 U/µl
• Contains 2 inert dyes (red and yellow) to facilitate PCR preparation and allow direct deposition on electrophoresis gel and migration monitoring
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 2 different 10X reaction buffers: without MgCl₂ (A) and with MgCl₂ (B)
• Kit supplied with dNTPs (separate tube)

Cytiva PCR components

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Taq DNA Polymerase (cloned): 1 unit incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 min at 70ºC, in a total volume of 50 µl.

Cytiva illustra™ PCR kits: Ready-To-Go™ technique

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PuReTaq Ready-To-Go PCR Beads are pre-mixed, predispensed, single-dose reactions optimised for performing standard PCR amplifications.

Cytiva PCR illustra™ kits: Ready-To-Go™ technique

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The RTG (Ready-To-Go) is to provide the mix necessary for the reaction as preformulated, premixed, predispensed beads

• Increased reproducibility
• Minimized pipetting steps
• Reduced the potential for contamination
• Long-term ambient-temperature stable (> 12 months)

Billes illustra Hot Start Mix RTG: pour les PCR nécessitant une haute spécificité de l'amplification. Les seuls réactifs to ajouter sont: l'eau, l'ADN to amplifier, les amorces.

La réaction Polymérase Chain Reaction (PCR) est couvert par des brevets de Roche Molecular Systems and F Hoffmann-La Roche Ltd.