Column Purification - Laboratoriumuitrusting

Cytiva illustra GFX™ PCR DNA and Gel Band Purification Kits

• This kit can be used to purify DNA from reaction volumes up to 100 µl or agarose gel slices up to 900 mg
• Recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution
• 99.5% of contaminants removed

Advantages:
• flexible 10 to 50 µl elution volume for different DNA concentration needs;
• Two choices of elution buffer;
• Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.

Purification and concentration of DNA fragments ranging in size from 50 bp to 10 kb, from:
• PCR mixtures;
• DNA-containing agarose gel bands;
• enzyme-based DNA modifications, and restriction enzyme digests

Genup kits

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• Fast and convenient
• High yield
• Purification of FGT PCR: binding / elution (no washing step)

Illustra MicroSpin HR columns

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Cytiva illustra GFX™ PCR* DNA and Gel Band Purification Kits

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• Can be used to purify DNA from reaction volumes up to 100 µl or agarose gel slices up to 900 mg
• Typical recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution
• 99.5% of contaminants are removed

Benefits:
• Flexible 10 to 50 µl elution volume for different DNA concentration needs;
• 2 choices of elution buffer;
• Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.

Rapid purification and concentration of DNA fragments (50 bp to 10 kb) from:
• PCR mixtures;
• DNA-containing agarose gel bands;
• enzyme-based DNA modifications, and restriction enzyme digests.

Purification of PCR products and fragments from NucleoSpin MACHEREY-NAGEL gel strips

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• Purification of DNA or RNA from agarose gels or polyacrylamide gels and purification of PCR products
• Silica membrane column purification by centrifugation
• For sample:
- < 400 µl PCR reaction mix, < 400 µg agarose gel for Mini kit
- < 4 ml of PCR reaction mixture , < 4 mg of agarose gel for Midi format kit
- < 10 ml of PCR reaction mixture , < 10 mg of agarose gel for Maxi format kit
• For fragment sizes from 50 bp to 20 kbp
• Elution volume:
- from 15 to 30 µl for the Mini kit
- from 200 to 400 µl for the Midi format kit
- 1 ml for the Maxi size kit

PCR product purification kit - Exo PCR ClearUP

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• Cleaning of PCR products in one step
• Protocole simple et rapide (5 min)
• Without the use of columns or magnetic balls
• Elimine les amorces résiduelles, l'ADN simple brin et inactive les dNTPs en excès par déphosphorylation
• Purified products for use in the preparation of NGS libraries