Transfer membranes for blotting

Protran NC nitrocellulose blotting membrane

• 100% pure nitrocellulose membrane 0.1 µm for molecules<7 kD
• High binding, low background
• High retention of small proteins:
• 0.2 µm membrane retains samples of less than 20 KD
• 0.45 µm membrane is designed for molecules over 20 KD
• Excellent stability of proteins on the Protran (5 years)
• No pre-wetting step with methanol: before the transfer, a simple wetting with water is enough
• Excellent stability of the membrane: allows many manipulations

Nytran SuPerCharge (SPC) nylon blotting membrane

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• Very high positive charge, low background
• Nytran SuperCharge membrane binds nine times more molecules than a conventional nylon membrane
• The great nylon density (compared to typical nylon membranes) provides increased binding sites for your samples
• Excellent sy mmetry: gives the membrane the ability to lie flat
• Very uniform pore size and pore distribution compared to typical nylon membranes: lead to greater reproducibility of results across a membrane and from blot to blot

Immobilon transfer membranes

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Molecular weight markers: Amersham ECL Plex™ fluorescent Rainbow™ Markers

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• optimised for use with the ECLPlex Western Blotting Detection System
• optimised to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels
• For proteins from 12 to 225 kDa
• RNP 850 E:
for 12 minigel loadings / RPN 851E: for 50 minigel loadings

Whatman blotting paper

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Nytran N nylon blotting membrane

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• Suitable for applications that require a lower charge
• Designed for Southern and Northern blotting, colony and plaque lifts and Dot-/Slot-Blots
• Compatible with isotopic and nonisotopic detection methods
• Excellent signal-to-noise ratios
• Moderate positive charge
• Consistent membrane morphology
• High sensitivity
• Nytran binding capacity: >400 µg/ cm²
• It is available in 0.2 µm and 0.45 µm pore sizes for optimal retention of oligos and larger DNA fragments

Amersham Protran nitrocellulose blotting membrane

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The Hybond and Whatman brands combine to give Amersham optimised blotting membranes.

Amersham Hybond PVDF blotting membrane

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• Ready-to-use solution for blotting applications
• Contains precut Amersham Hybond P 0.2 µm PVDF membranes preassembled with 2 x 3 mm Chr Blotting papers
• The orientation of the sandwich must be so that the membrane is on the anode side of the gel
• 3 mm Chr paper sheets cover each side of the sandwich
• The gel is ready for transfer

Amersham™ HYBOND blotting membrane

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Hybond-N
• Strong, supported membrane
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Recommended for radioactive blotting
• Nucleic acids can be crosslinked to nylon using UV ligh

Hybond-N+
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• For use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems

Hybond-NX
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Developed specifically for use with low hybridization buffer volumes and high-throughput applications
• Gives clean backgrounds

Hybond-XL
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Efficient binding of small fragments
• Positively charged nylon membrane which is designed to give optimum signal-to-noise ratios when used with radioactively labeled probes

More dimensions available: please contact us.

Amersham ECL and MP Hyperfilm™

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For detection of the chemiluminescence signal of the nucleic acid and protein blots.

TurboBlotter: rapid blotting device for transfer of DNA and RNA.

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• Uses capilarity combined with gravity
• Compact: small footprint, stackable
• Blotting device for high-resolution transfer of DNA and RNA
• Rapid: downward capillary transfer allows for alkaline buffer transfers in 1 h and neutral (SSC) transfers in 3 h
• Economical: Reusable blotting device requires less buffer and blotting paper
• Easy to use: Very easy to set up and works without power or vacuum source

Each TurboBlotter Kit contains:
• 1 transfer device;
• membranes and papers, enough for five transfers

Each TurboBlotter refill contains five sets of membranes and papers, enough for five transfers.

Protran NC nitrocellulose blotting membrane

Nytran SuPerCharge (SPC) nylon blotting membrane

Immobilon transfer membranes

Molecular weight markers: Amersham ECL Plex™ fluorescent Rainbow™ Markers

Whatman blotting paper

Nytran N nylon blotting membrane

Amersham Protran nitrocellulose blotting membrane

Amersham Hybond PVDF blotting membrane

Amersham™ HYBOND blotting membrane

Amersham ECL and MP Hyperfilm™

TurboBlotter: rapid blotting device for transfer of DNA and RNA.

ECL Prime blocking reagent

Amersham ECL™, ECL Start™, ECL Advance™, ECL Select™, ECL Prime

ECL blocking agent

Hybond ECL Membranes