Thermosensitive bacterial alkaline phosphatase (BAP)

• Very suitable for diagnostic immunodetection techniques and proteins immunolabelling and nucleic acids transferred on membrane techniques
• Catalyses the release of 5'-P and 3'-P groups of DNA, RNA and nucleotide molecules
• Removes the 5'-P from DNA, RNA, rNTP and dNTP molecules
• Used to remove the 5'-P ends of linearized vectors to prevent their "empty" recircularization during cloning processes
• Resistant to chemical changes and active in a wide variety of reaction buffers
• Can be inactivated by heat for 5 minutes at 70 °C
• Compatible with protein dephosphorylation
• Available in 1000 and 5000 unit formats