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GSTrap™ columns

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GE Chromatography Guide
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Variants and accessories

GSTrap™ columnsOther formatsCleavage enzyme
GSTrap columns are designed for one-step purification of GST-tagged proteins. The columns are prepacked with the matrices:
• Glutathion Sepharose HP: for high-resolution purifications, from a more concentrated sample;
• Glutathion Sepharose FF: for high flow rate purifications;
• Glutathion Sepharose 4B, standard support.

Available in 2 formats: 1 or 5 ml column.
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GSTrap HP, volume: 5 x 1 ml
Sales Unit : 1
GSTrap HP, volume: 1 x 5 ml
Sales Unit : 1
GSTrap HP, volume: 5 x 5 ml
Sales Unit : 1
GSTrap FF, volume: 2 x 1 ml
Sales Unit : 1
GSTrap FF, volume: 5 x 1 ml
Sales Unit : 1
GSTrap FF, volume: 1 x 5 ml
Sales Unit : 1
GSTrap FF, volume: 5 x 5 ml
Sales Unit : 1
GSTrap 4B, volume: 5 x 1 ml
Sales Unit : 1
GSTrap 4B, volume: 1 x 5 ml
Sales Unit : 1
GSTrap 4B, volume: 5 x 5 ml
Sales Unit : 1
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GSTPrep FF 16/10
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GST fusion protein cleavage enzyme

Used for site-specific separation of the GST tag from proteins:
• expressed using pGEX-T vectors: Thrombin Protease;
• expressed using pGEX-X vectors: Facto Xa Protease;
• expressed using pGEX-6P vectors: PreScission™ Protease.

PreScission Protease: one unit will cleave >90 % of 100 µg of a test GST-fusion protein in 50 mm Tris-HCl, 150 mm NaCl, 1 mm EDTA, 1 mm DTT, pH 7.0 at 5 °C for 16 h.
Thrombin Protease: one unit of enzyme cleaves 90% of 100 µg of a test GST fusion protein when incubated in 1 × PBS at 22 °C for 16 h
Factor Xa Protease: one unit cleaves >90 % of 100 µg of a test GST fusion protein when incubated in 1 mm CaCl₂, 100 mm NaCl, and 50 mm Tris-HCl (pH 8.0) at 22 °C for 16 h.
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Prescission™ protease - 2000 units/ml in 50 mM Tris-HCl, 150 mM NaCl, 10mM EDTA, 1 mM DTT and 20 % glycérol)
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Specific transport
Item concerned by specific transport and/or packaging costs
Thrombin protease, lyophilized powder 500 UI
Sales Unit : 1
Specific transport
Item concerned by specific transport and/or packaging costs

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Specific terms
The sales prices displayed on this page are expressed without tax, those valid at the time of consultation, and take precedence over any price visible in a paper catalogue or in a PDF document.
The indication of the stock is not contractual: this data is in constant evolution and can vary according to the desired quantities.
*Item available on special supply: please contact us for delivery times
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