Hot Start DNA polymerases - Laboratoriumuitrusting

tiAmplus DNA Polymerase

• Optimized mixture of thermostable DNA polymerases Taq (Thermus aquaticus), Pfu (Pyrococcus furiosus) and a polymerization activator
• Ultrapure recombinant polymerases, thermostable and hot start. Pfu proofreading
• The hot start property of the polymerases makes them inactive at room temperature allowing the preparation of the reaction mix at room temperature
• Unique composition of the reaction buffer promoting pH stability at high temperature and ensuring high efficiency for long sequence amplifications
• Amplification of genomic DNA sequences from 3 to 25 kb and episomal DNA sequences up to 40 kb
• Particularly suitable for the amplification of eukaryotic genomes
• Ideal for genome mapping and sequencing of long fragments
• Supplied with a 10X reaction buffer
• Available in 100 and 500 unit formats

Cytiva Hot Start MasterMix

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• For demanding PCR applications in which high specificity are essential
• Reduces nonspecific priming
• Kit Contents: Tris-HCl, KCl, MgCl₂, dNTPs, Taq DNA Polymerase, Hot Start Activator protein, additional MgCl₂ tube

Cytiva PCR illustra™ kits: Ready-To-Go™ technique

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The RTG (Ready-To-Go) is to provide the mix necessary for the reaction as preformulated, premixed, predispensed beads

• Increased reproducibility
• Minimized pipetting steps
• Reduced the potential for contamination
• Long-term ambient-temperature stable (> 12 months)

Billes illustra Hot Start Mix RTG: pour les PCR nécessitant une haute spécificité de l'amplification. Les seuls réactifs to ajouter sont: l'eau, l'ADN to amplifier, les amorces.

La réaction Polymérase Chain Reaction (PCR) est couvert par des brevets de Roche Molecular Systems and F Hoffmann-La Roche Ltd.

PAXgene Blood sampling Tubes

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• For the stabilization of DNA, RNA or circulating, cell-free DNA (ccfDNA) depending on Cat.No.
• Secure and standardized sampling
• CE/IVD certified
• Compatible with sensitive applications: RT-qPCR, NGS, methylation...

OnTaq DNA Polymerase Hot Start Kit (2.5 U/µl) with dNTPs

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• DNA polymerase hot start at an initial concentration of 2.5 U/µl
• Increases PCR specificity, sensitivity and throughput by reducing the risk of mismatch and primer-dimer formation
• Activated during the initial 10 min denaturation step at 95 °C
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl₂ (A), with MgCl₂ (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

Master mix Fast Sybr Green qPCR (2X) and Master mix Fast SG qPCR (2X) with ROX solution

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• Fast qPCR mix with SYBR Green I dye for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer, dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix Fast Probe qPCR (2X) and Master mix Fast Probe qPCR (2X) with ROX solution

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• Fast qPCR mix with hydrolysis probes for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer, dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix HRM PCR (2X)

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• Mix designed for high resolution melting curve (HRM) analysis of DNA samples
• Allows detection of gene mutations and single polymorphisms (SNPs) including class III and IV
• Contains onTaq hot start DNA polymerase, optimised buffer, dNTPs and fluorescent dye
• OnTaq polymerase activated during the initial denaturation step of the PCR at 95 °C for 15 min